thrD [2019-05-07 15:41:32]

aspartokinase III

locus: BSU03790

pI: 4.78

mw: 49.66 kDa

protein length: 454 aa

gene length: 1365 bp

function: unknown

product: aspartokinase III

essential: no

ec: 2.7.2.4

synonyms: yclM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]

Gene

Coordinates: 430,623 431,987

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate = ADP + 4-phospho-L-aspartate (according to Swiss-Prot)

Protein family

• aspartokinase family (according to Swiss-Prot)

[SW|Domains]

• [SW|ACT domain] (aa 389 ... 454) (according to the Interpro database)

Effectors of protein activity

• inhibited by the simultaneous presence of threonine and lysine [Pubmed|11471740]

Structure

• [PDB|3TVI] (from Clostridium acetobutylicum, 37% identity) [pubmed|25170437]

Expression and Regulation

Operons

• genes: [gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|23569278], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR]: repression, [Pubmed|27260660], in [regulon|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR regulon]

  regulation

  • expressed in the presence of lysine or cysteine ([SW|ThrR]) [Pubmed|27260660]

Biological materials

Mutant

• MGNA-C009 (yclM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2007 NBRP B. subtilis, Japan]
• BKE03790 ([gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG
• BKK03790 ([gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACATCTCCTAATG, downstream forward: _UP4_TAATCGTACATAAATAGCGG

lacZ fusion

• pBP318 (in [SW|pAC5]), available in [SW|Fabian Commichau]'s lab [Pubmed|27260660]

References

Reviews

• 19946135,22079167

Original publications

• 11471740,23569278,2152900,2153658,22383849,27260660,25170437